A comparison of three Peyer's patch "M-like" cell culture models: particle uptake, bacterial interaction, and epithelial histology

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dc.contributor.author Tauseef Ahmad, Muhammad‏
dc.contributor.author Gogarty, Martina
dc.contributor.author Walsh, Edwin G.
dc.contributor.author Brayden, David James
dc.date.accessioned 2017-09-13T09:22:47Z
dc.date.copyright 2017 Elsevier en
dc.date.issued 2017-10
dc.identifier.citation European journal of pharmaceutics and biopharmaceutics en
dc.identifier.uri http://hdl.handle.net/10197/8762
dc.description.abstract Intestinal Peyer's patch (PP) microfold (M) cells transport microbes and particulates across the follicle-associated epithelium (FAE) as part of the mucosal immune surveillance system. In vitro human M-like cell co-culture models are used as screens to investigate uptake of antigens-in-nanoparticles, but the models are labour-intensive and there is inter-laboratory variability. We compared the three most established filter-grown Caco-2/Raji B cell co-culture systems. These were Model A (Kerneis et al, 1997), Model B (Gullberg et al., 2000), and Model C (Des Rieux et al. 2007). The criteria used were transepithelial resistance (TEER), the apparent permeability coefficient (Papp) of [(14)C]-mannitol, M cell-like histology, as well as latex particle and Salmonella typhimurium translocation. Each co-culture model displayed substantial increases in particle translocation. Truncated microvilli compared to mono-cultures was their most consistent feature. The inverted model developed by des Rieux et al. (2007) displayed reductions in TEER and an increased (Papp), accompanied by the largest increase in particle translocation compared to the other two models. The normally-oriented model developed by Gullberg et al. (2000) was the only one to consistently display an increased translocation of salmonella typhimurium. By applying a double Matrigel¿¿¿ coating on filters, altering the medium feeding regime for Raji B cells, and restricting the passage number of B cells, improvements to the Gullberg model B were achieved, as reflected by increased particle translocation and improved histology. In conclusion, this is the first time all three designs have been compared in one study and each displays phenotypic features of M-like cells. While Model C was the most robust co-culture, the Model B protocol could be improved by optimizing several variables and is less complicated to establish than the two inverted models.. en
dc.description.sponsorship Science Foundation Ireland en
dc.language.iso en en
dc.publisher Elsevier en
dc.rights This is the author’s version of a work that was accepted for publication in European journal of pharmaceutics and biopharmaceutics. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in European journal of pharmaceutics and biopharmaceutics, 119 2017-10, pp.426-436. DOI: 10.1016/j.ejpb.2017.07.013 en
dc.subject Peyer’s patch M cells en
dc.subject M-like cells en
dc.subject Caco-2 en
dc.subject Nanoparticle en
dc.subject Follicle-associated epithelium en
dc.subject Oral vaccine delivery en
dc.title A comparison of three Peyer's patch "M-like" cell culture models: particle uptake, bacterial interaction, and epithelial histology en
dc.type Journal Article en
dc.internal.authorcontactother david.brayden@ucd.ie
dc.status Peer reviewed en
dc.identifier.volume 119 en
dc.identifier.startpage 426 en
dc.identifier.endpage 436 en
dc.identifier.doi 10.1016/j.ejpb.2017.07.013
dc.neeo.contributor Tauseef Ahmad|Muhammad‏|aut|
dc.neeo.contributor Gogarty|Martina|aut|
dc.neeo.contributor Walsh|Edwin G.|aut|
dc.neeo.contributor Brayden|David James|aut|
dc.date.embargo 2018-07-25
dc.internal.rmsid 784255494
dc.date.updated 2017-07-31T10:49:19Z
 Access to this item has been restricted by the copyright holder until: 2018-07-25

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