Effects of the Presence or Absence of a Protein Corona on Silica Nanoparticle Uptake and Impact on Cells

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dc.contributor.author Lesniak, Anna
dc.contributor.author Fenaroli, Federico
dc.contributor.author Monopoli, Marco P.
dc.contributor.author Åberg, Christoffer
dc.contributor.author Dawson, Kenneth A.
dc.contributor.author Salvati, Anna
dc.date.accessioned 2012-10-02T14:30:06Z
dc.date.copyright 2012 American Chemical Society en
dc.date.issued 2012-06-21
dc.identifier.citation ACS Nano en
dc.identifier.uri http://hdl.handle.net/10197/3830
dc.description.abstract Nanoparticles enter cells through active processes, thanks to their capability of interacting with the cellular machinery. The protein layer (corona) that forms on their surface once nanoparticles are in contact with biological fluids, such as the cell serum, mediates the interactions with cells in situ. As a consequence of this, here we show that the same nanomaterial can lead to very different biological outcomes, when exposed to cells in the presence or absence of a preformed corona. In particular, silica nanoparticles exposed to cells in the absence of serum have a stronger adhesion to the cell membrane and higher internalization efficiency, in comparison to what is observed in medium containing serum, when a preformed corona is present on their surface. The different exposure conditions not only affect the uptake levels but also result in differences in the intracellular nanoparticle location and impact on cells. Interestingly, we also show that after only one hour of exposure, a corona of very different nature forms on the nanoparticles exposed to cells in the absence of serum. Evidence suggests that these different outcomes can all be connected to the different adhesion and surface properties in the two conditions. en
dc.description.sponsorship Irish Research Council for Science, Engineering and Technology en
dc.description.sponsorship European Research Council en
dc.language.iso en en
dc.publisher ACS Publications en
dc.relation.requires Centre for Bionano Interactions (CBNI) Research Collection en
dc.subject Nanoparticle en
dc.subject Silica en
dc.subject Serum free en
dc.subject Protein corona en
dc.subject Nanoparticle uptake en
dc.subject Adhesion en
dc.subject.lcsh Nanoparticles en
dc.subject.lcsh Silica en
dc.subject.lcsh Serum-free culture media en
dc.subject.lcsh Adhesion en
dc.title Effects of the Presence or Absence of a Protein Corona on Silica Nanoparticle Uptake and Impact on Cells en
dc.type Journal Article en
dc.internal.availability Full text available en
dc.status Peer reviewed en
dc.identifier.volume 6 en
dc.identifier.issue 7 en
dc.identifier.startpage 5845 en
dc.identifier.endpage 5857 en
dc.identifier.doi 10.1021/nn300223w
dc.neeo.contributor Lesniak|Anna|aut|
dc.neeo.contributor Fenaroli|Federico|aut|
dc.neeo.contributor Monopoli|Marco P.|aut|
dc.neeo.contributor Åberg|Christoffer|aut|
dc.neeo.contributor Dawson|Kenneth A.|aut|
dc.neeo.contributor Salvati|Anna|aut|

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