Purification and properties of fluoroacetate dehalogenase from Pseudomonas fluorescens DSM 8341

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dc.contributor.author Donnelly, Clár
dc.contributor.author Murphy, Cormac D.
dc.date.accessioned 2012-08-17T14:18:59Z
dc.date.available 2012-08-17T14:18:59Z
dc.date.copyright 2008 Springer Science+Business Media B.V. en
dc.date.issued 2009
dc.identifier.citation Biotechnology Letters en
dc.identifier.uri http://hdl.handle.net/10197/3745
dc.description.abstract The degradation of fluoroacetate by microorganisms has been established for some time, although only a handful of dehalogenases capable of hydrolyzing the stable C-F bond have been studied. The bacterium Pseudomonas fluorescens DSM 8341 was originally isolated from soil and very readily degraded fluoroacetate, thus it was thought that its dehalogenase might have some desirable properties. The enzyme was purified from cell free extracts and characterised: it is a monomer of 32,500 Da, with a pH optimum of 8 and is stable between pH 4 and 10; its activity is stimulated by some metal ions (Mg2+, Mn2+ and Fe3+), but inhibited by others (Hg2+, Ag2+). The enzyme is specific for fluoroacetate, and the Km for this substrate (0.68 mM) is the lowest determined for enzymes of this type that have been investigated to date. en
dc.description.sponsorship Other funder en
dc.format.extent 411648 bytes
dc.format.mimetype application/msword
dc.language.iso en en
dc.publisher Springer en
dc.relation.requires CSCB Research Collection en
dc.rights The final publication is available at www.springerlink.com en
dc.subject Dehalogenation en
dc.subject Fluoroacetate en
dc.subject Fluorocitrate en
dc.subject Pseudomonas fluorescens en
dc.subject.lcsh Halogen compounds en
dc.subject.lcsh Organofluorine compounds--Purification en
dc.subject.lcsh Pseudomonas fluorescens en
dc.title Purification and properties of fluoroacetate dehalogenase from Pseudomonas fluorescens DSM 8341 en
dc.type Journal Article en
dc.internal.availability Full text available en
dc.status Peer reviewed en
dc.identifier.volume 31 en
dc.identifier.issue 2 en
dc.identifier.startpage 245 en
dc.identifier.endpage 250 en
dc.identifier.doi 10.1007/s10529-008-9849-4
dc.neeo.contributor Donnelly|Clár|aut| en
dc.neeo.contributor Murphy|Cormac D.|aut| en
dc.description.othersponsorship Enterprise Ireland en

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