Experimental and theoretical approach to comparative nanoparticle and small molecule intracellular import, trafficking, and export
Date:
2011-12
Recommended citation:
Salvati, Anna, Åberg, Christoffer, dos Santos, Tiago, Varela, Juan, Pinto, Paulo, Lynch, Iseult, Dawson, Kenneth A.
: Experimental and theoretical approach to comparative nanoparticle and small molecule intracellular import, trafficking, and export. Nanomedicine: Nanotechnology, Biology and Medicine, 7 (6) 2011-12, pp.818-826.
Abstract:
Central to understanding how nanoscale objects interact with living matter is the need for reproducible and verifiable data that can be interpreted with confidence. Likely this will be the basis of durable advances in nanomedicine and nanomedical safety. To develop these fields, there is also considerable interest in advancing the first generation of theoretical models of nanoparticle (NP) uptake into cells, and NP biodistribution in general. Here we present an uptake study comparing the outcomes for free molecular dye and NPs labeled with the same dye. A simple flux-based approach is presented to model NP uptake. We find that the intracellular NP concentration grows linearly in time, and that the uptake is essentially irreversible, with the particles accumulating in lysosomes. A wide range of practical challenges, from labile dye release to NP aggregation and the need to account for cell division, are addressed to ensure that these studies yield meaningful kinetic information.
Date:
Experimental and theoretical comparison of intracellular import of polymeric nanoparticles and small molecules : toward models of uptake kinetics
Funding Details:
Science Foundation Ireland; Irish Research Council for Science, Engineering and Technology
Funding Details:
European Commission (FP6)
Type of material:
Journal Article
Publisher:
Elsevier
Copyright (published version):
2011 Elsevier Inc.
Rights statement:
This is the author’s version of a work that was accepted for publication in Nanomedicine: Nanotechnology, Biology and Medicine, Volume 7, Issue 6, December 2011, Pages 818-826. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version will be subsequently published in Nanomedicine: Nanotechnology, Biology and Medicine DOI: 10.1016/j.nano.2011.03.005
ISSN:
1549-9634
Status of item:
Peer reviewed
Language:
en
Availability:
Full text available
Available:
2011-11-24T15:07:23Z
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