Factors influencing 4-fluorobenzoate degradation in biofilm cultures of Pseudomonas knackmussii B13

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dc.contributor.author Misiak, Katarzyna
dc.contributor.author Casey, Eoin
dc.contributor.author Murphy, Cormac D.
dc.date.accessioned 2011-05-30T15:17:56Z
dc.date.available 2011-05-30T15:17:56Z
dc.date.copyright 2011 Elsevier Ltd en
dc.date.issued 2011-05
dc.identifier.citation Water Research en
dc.identifier.issn 0043-1354
dc.identifier.uri http://hdl.handle.net/10197/2960
dc.description.abstract Membrane aerated biofilm reactors (MABRs) have potential in wastewater treatment as they permit simultaneous COD minimisation, nitrification and denitrification. Here we report on the application of the MABR to the removal of fluorinated xenobiotics from wastewater, employing a Pseudomonas knackmussii monoculture to degrade the model compound 4-fluorobenzoate. Growth of biofilm in the MABR using the fluorinated compound as the sole carbon source occurred in two distinct phases, with early rapid growth (up to 0.007 h−1) followed by ten-fold slower growth after 200 h operation. Furthermore, the specific 4-fluorobenzoate degradation rate decreased from 1.2 g g−1 h−1 to 0.2 g g−1 h−1, indicating a diminishing effectiveness of the biofilm as thickness increased. In planktonic cultures stoichiometric conversion of substrate to the fluoride ion was observed, however in the MABR, approximately 85% of the fluorine added was recovered as fluoride, suggesting accumulation of ‘fluorine’ in the biofilm might account for the decreasing efficiency. This was investigated by culturing the bacterium in a tubular biofilm reactor (TBR), revealing that there was significant fluoride accumulation within the biofilm (0.25 M), which might be responsible for inhibition of 4-fluorobenzoate degradation. This contention was supported by the observation of the inhibition of biofilm accumulation on glass cover slips in the presence of 40 mM fluoride. These experiments highlight the importance of fluoride ion accumulation on biofilm performance when applied to organofluorine remediation. en
dc.description.sponsorship Higher Education Authority en
dc.format.extent 721536 bytes
dc.format.mimetype application/pdf
dc.language.iso en en
dc.publisher Elsevier en
dc.relation.requires Biomolecular and Biomedical Science Research Collection en
dc.rights This is the author’s version of a work that was accepted for publication in Water Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Water Research Volume 45, Issue 11, May 2011, Pages 3512-3520 DOI: 10.1016/j.watres.2011.04.020 en
dc.subject Biofilm en
dc.subject Membrane en
dc.subject Fluorobenzoate en
dc.subject Preudomonas en
dc.subject Fluoride en
dc.subject Biodegradation en
dc.subject.lcsh Biofilms en
dc.subject.lcsh Organofluorine compounds en
dc.subject.lcsh Pseudomonas en
dc.subject.lcsh Biodegradation en
dc.title Factors influencing 4-fluorobenzoate degradation in biofilm cultures of Pseudomonas knackmussii B13 en
dc.type Journal Article en
dc.internal.availability Full text available en
dc.internal.webversions http://dx.doi.org/10.1016/j.watres.2011.04.020 en
dc.status Peer reviewed en
dc.identifier.volume 45 en
dc.identifier.issue 11 en
dc.identifier.startpage 3512 en
dc.identifier.endpage 3520 en
dc.identifier.doi 10.1016/j.watres.2011.04.020
dc.neeo.contributor Misiak|Katarzyna|aut| en
dc.neeo.contributor Casey|Eoin|aut| en
dc.neeo.contributor Murphy|Cormac D.|aut| en

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