Characterisation of a modified rotating disk reactor for the cultivation of Staphylococcus epidermidis biofilm

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dc.contributor.author Cotter, John J.
dc.contributor.author O'Gara, James P.
dc.contributor.author Stewart, Philip S.
dc.contributor.author Pitts, Betsey
dc.contributor.author Casey, Eoin
dc.date.accessioned 2011-01-24T17:31:30Z
dc.date.available 2011-01-24T17:31:30Z
dc.date.copyright 2010 The Authors; Journal of Applied Microbiology; The Society for Applied Microbiology en
dc.date.issued 2010-12
dc.identifier.citation Journal of Applied Microbiology en
dc.identifier.issn 1365-2672
dc.identifier.uri http://hdl.handle.net/10197/2745
dc.description.abstract Aims:  The purpose of this study was to develop a system that would allow biofilms to be cultivated under strictly defined conditions in terms of dissolved oxygen, fluid shear and to assess whether the method was suitable for the detection of respiratory activity stratification in biofilm samples. Methods:  The system is a modified version a commercially available laboratory biofilm reactor and incorporates a number of features such as the provision of defined levels of dissolved oxygen, constant average shear, enhanced gas–liquid mass transfer, aseptic operation and the ability to remove biofilm for ex situ analysis during or after continuous cultivation. Conclusions:  The system was shown to be effective for the characterization of the effects of dissolved oxygen on a pure culture of Staphylococcus epidermidis. The versatility of the system offers the potential for cultivating pure culture biofilm in defined, controlled conditions and facilitates a range of analyses that can be performed ex situ. Significance and Impact of the Study:  The ability to provide strict regulation of environmental conditions and enhanced transfer of oxygen to the biofilm during cultivation are important, first because oxygen is known to regulate biofilm development in several micro-organisms and second because many conventional biofilm cultivation systems may not provide adequate oxygen supply to the biofilm. en
dc.description.sponsorship Science Foundation Ireland en
dc.format.extent 403033 bytes
dc.format.mimetype application/pdf
dc.language.iso en en
dc.publisher Wiley en
dc.relation.requires Conway Institute Research Collection en
dc.relation.requires CSCB Research Collection en
dc.rights This is the authors' version of the following article: "Characterisation of a modified Rotating Disk Reactor for the cultivation of Staphylococcus epidermidis biofilm" published in Journal of Applied Microbiology, 109 (6): 2105-2117. It is available in its final form at http://dx.doi.org/10.1111/j.1365-2672.2010.04842.x en
dc.subject Biofilm en
dc.subject Reactor en
dc.subject Oxygen en
dc.subject Cultivation en
dc.subject Sensor en
dc.subject Antibiotic en
dc.subject Shear en
dc.subject Staphylococcus en
dc.subject.lcsh Biofilms en
dc.subject.lcsh Antibiotics en
dc.subject.lcsh Staphylococcus en
dc.subject.lcsh Oxygen en
dc.title Characterisation of a modified rotating disk reactor for the cultivation of Staphylococcus epidermidis biofilm en
dc.type Journal Article en
dc.internal.availability Full text available en
dc.internal.webversions Publisher's version en
dc.internal.webversions http://dx.doi.org/10.1111/j.1365-2672.2010.04842.x en
dc.status Peer reviewed en
dc.identifier.volume 109 en
dc.identifier.issue 6 en
dc.identifier.startpage 2105 en
dc.identifier.endpage 2117 en
dc.identifier.doi 10.1111/j.1365-2672.2010.04842.x
dc.neeo.contributor Cotter|John J.|aut| en
dc.neeo.contributor O'Gara|James P.|aut| en
dc.neeo.contributor Stewart|Philip S.|aut| en
dc.neeo.contributor Pitts|Betsey|aut| en
dc.neeo.contributor Casey|Eoin|aut| en


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