Rapid depletion of dissolved oxygen in 96 well microtitre plate Staphylococcus epidermidis biofilm assays promotes biofilm development and is influenced by inoculum cell concentration

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dc.contributor.author Cotter, John J.
dc.contributor.author O'Gara, James P.
dc.contributor.author Casey, Eoin
dc.date.accessioned 2011-01-19T14:27:44Z
dc.date.available 2011-01-19T14:27:44Z
dc.date.copyright 2009 Wiley Periodicals, Inc. en
dc.date.issued 2009-08-01
dc.identifier.citation Biotechnology and Bioengineering en
dc.identifier.issn 1097-0290
dc.identifier.uri http://hdl.handle.net/10197/2729
dc.description.abstract Biofilm-related research using 96-well microtiter plates involves static incubation of plates indiscriminate of environmental conditions, making oxygen availability an important variable which has not been considered to date. By directly measuring dissolved oxygen concentration over time we report here that dissolved oxygen is rapidly consumed in Staphylococcus epidermidis biofilm cultures grown in 96-well plates irrespective of the oxygen concentration in the gaseous environment in which the plates are incubated. These data indicate that depletion of dissolved oxygen during growth of bacterial biofilm cultures in 96-well plates may significantly influence biofilm production. Furthermore higher inoculum cell concentrations are associated with more rapid consumption of dissolved oxygen and higher levels of S. epidermidis biofilm production. Our data reveal that oxygen depletion during bacterial growth in 96-well plates may significantly influence biofilm production and should be considered in the interpretation of experimental data using this biofilm model. en
dc.description.sponsorship Science Foundation Ireland en
dc.format.extent 142748 bytes
dc.format.mimetype application/pdf
dc.language.iso en en
dc.publisher Wiley en
dc.relation.requires Conway Institute Research Collection en
dc.rights This is the authors' version of the following article: "Rapid depletion of dissolved oxygen in 96-well microtiter plate Staphylococcus epidermidis biofilm assays promotes biofilm development and is influenced by inoculum cell concentration" published in Biotechnology and Bioengineering 103:5 pp 1042–1047. It is available in its final form at http://dx.doi.org/10.1002/bit.22335 en
dc.subject Biofilm en
dc.subject Oxygen en
dc.subject Staphylococcus epidermidis en
dc.subject Microtitre en
dc.subject 96-well plate en
dc.subject.lcsh Biofilms en
dc.subject.lcsh Oxygen en
dc.subject.lcsh Staphylococcus en
dc.subject.lcsh Microplates en
dc.title Rapid depletion of dissolved oxygen in 96 well microtitre plate Staphylococcus epidermidis biofilm assays promotes biofilm development and is influenced by inoculum cell concentration en
dc.type Journal Article en
dc.internal.availability Full text available en
dc.internal.webversions Publisher's version en
dc.internal.webversions http://dx.doi.org/10.1002/bit.22335 en
dc.status Peer reviewed en
dc.identifier.volume 103 en
dc.identifier.issue 5 en
dc.identifier.startpage 1042 en
dc.identifier.endpage 1047 en
dc.identifier.doi 10.1002/bit.22335
dc.neeo.contributor Cotter|John J.|aut| en
dc.neeo.contributor O'Gara|James P.|aut| en
dc.neeo.contributor Casey|Eoin|aut| en


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