College of Sciencehttp://hdl.handle.net/10197/179720140902T23:51:53Z20140902T23:51:53ZIdentification of a mutation in LARS as a novel cause of infantile hepatopathyCasey, JillianMcGettigan, Paul A.LynamLennon, NiamhRegan, ReginaConroy, JudithBourke, BillyLynch, SallyEnnis, Seanet al.http://hdl.handle.net/10197/579420140807T11:03:03Z20120701T00:00:00ZIdentification of a mutation in LARS as a novel cause of infantile hepatopathy
Casey, Jillian; McGettigan, Paul A.; LynamLennon, Niamh; Regan, Regina; Conroy, Judith; Bourke, Billy; Lynch, Sally; Ennis, Sean; et al.
Infantile hepatopathies are lifethreatening liver disorders that manifest in the first few months of life. We report on a consanguineous Irish Traveller family that includes six individuals presenting with acute liver failure in the first few months of life. Additional symptoms include anaemia, renal tubulopathy, developmental delay, seizures, failure to thrive and deterioration of liver function with minor illness. The multisystem manifestations suggested a possible mitochondrial basis to the disorder. However, known causes of childhood liver failure and mitochondrial disease were excluded in this family by biochemical, metabolic and genetic analyses. We aimed to identify the underlying risk gene using homozygosity mapping and whole exome sequencing. SNP homozygosity mapping identified a candidate locus at 5q31.3–q33.1. Whole exome sequencing identified 1 novel homozygous missense mutation within the 5q31.3–q33.1 candidate region that segregated with the hepatopathy. The candidate mutation is located in the LARS gene which encodes a cytoplasmic leucyltRNA synthetase enzyme responsible for exclusively attaching leucine to its cognate tRNA during protein translation. Knockdown of LARS in HEK293 cells did not impact on mitochondrial function even when the cells were put under physiological stress. The molecular studies confirm the findings of the patients' biochemical and genetic analyses which show that the hepatopathy is not a mitochondrialbased dysfunction problem, despite clinical appearances. This study highlights the clinical utility of homozygosity mapping and exome sequencing in diagnosing recessive liver disorders. It reports mutation of a cytoplasmic aminoacyltRNA synthetase enzyme as a possible novel cause of infantile hepatopathy and underscores the need to consider mutations in LARS in patients with liver disease and multisystem presentations.
20120701T00:00:00ZFirst implication of STRA6 mutations in isolated anophthalmia, microphthalmia and coloboma: a new dimension to the STRA6 phenotypeCasey, JillianKawaguchi, RikiMorrissey, MariaMcGettigan, Paul A.Nielsen, Jens ErikConroy, JudithRegan, ReginaKennedy, BreandánLynch, SallyGreen, AndrewEnnis, Seanet al.http://hdl.handle.net/10197/579020140807T09:32:55Z20111201T00:00:00ZFirst implication of STRA6 mutations in isolated anophthalmia, microphthalmia and coloboma: a new dimension to the STRA6 phenotype
Casey, Jillian; Kawaguchi, Riki; Morrissey, Maria; McGettigan, Paul A.; Nielsen, Jens Erik; Conroy, Judith; Regan, Regina; Kennedy, Breandán; Lynch, Sally; Green, Andrew; Ennis, Sean; et al.
Microphthalmia, anophthalmia, and coloboma (MAC) are structural congenital eye malformations that cause a significant proportion of childhood visual impairments. Several disease genes have been identified but do not account for all MAC cases, suggesting that additional risk loci exist. We used single nucleotide polymorphism (SNP) homozygosity mapping (HM) and targeted nextgeneration sequencing to identify the causative mutation for autosomal recessive isolated colobomatous microanophthalmia (MCOPCB) in a consanguineous Irish Traveller family. We identified a doublenucleotide polymorphism (g.1157G>A and g.1156G>A; p.G304K) in STRA6 that was homozygous in all of the MCOPCB patients. The STRA6 p.G304K mutation was subsequently detected in additional MCOPCB patients, including one individual with MatthewWood syndrome (MWS; MCOPS9). STRA6 encodes a transmembrane receptor involved in vitamin A uptake, a process essential to eye development and growth. We have shown that the G304K mutant STRA6 protein is mislocalized and has severely reduced vitamin A uptake activity. Furthermore, we reproduced the MCOPCB phenotype in a zebrafish disease model by inhibiting retinoic acid (RA) synthesis, suggesting that diminished RA levels account for the eye malformations in STRA6 p.G304K patients. The current study demonstrates that STRA6 mutations can cause isolated eye malformations in addition to the congenital anomalies observed in MWS.
20111201T00:00:00ZInterpreting map usage patterns using geovisual analytics and spatiotemporal clusteringMcArdle, GavinTahir, AliBertolotto, Michelahttp://hdl.handle.net/10197/570420140724T12:12:12Z20140301T00:00:00ZInterpreting map usage patterns using geovisual analytics and spatiotemporal clustering
McArdle, Gavin; Tahir, Ali; Bertolotto, Michela
Extracting meaningful information from the growing quantity of spatial data is a challenge. The issues are particularly evident with spatiotemporal data describing movement. Such data typically corresponds to movement of humans, animals and machines in the physical environment. This article considers a special form of movement data generated through human–computer interactions with online web maps. As a user interacts with a web map using a mouse as a pointing tool, invisible trajectories are generated. By examining the spatial features on the map where the mouse cursor visits, a user's interests and experience can be detected. To analyse this valuable information, we have developed a geovisual analysis tool which provides a rich insight into such user behaviour. The focus of this paper is on a clustering technique which we apply to mouse trajectories to group trajectories with similar behavioural properties. Our experiments reveal that it is possible to identify experienced and novice users of web mapping environments using an incremental clustering approach. The results can be used to provide personalised map interfaces to users and provide appropriate interventions for completing spatial tasks.
20140301T00:00:00ZHow to (Possibly) Foil Multimedia Security?Balado, FélixFournel, Thierryhttp://hdl.handle.net/10197/570020140724T11:58:56Z20140701T00:00:00ZHow to (Possibly) Foil Multimedia Security?
Balado, Félix; Fournel, Thierry
Multimedia security can be foiled thanks to
Slepian’s permutation modulation. Originally proposed in 1965
for standard problems of channel and source coding in communications,
permutation codes can also provide optimum solutions
in two relevant fields: steganography (foiling hidden information
detection tests) and counterforensics (foiling forensic detection
tests). In the first scenario, permutation codes have been shown
to implement optimum perfect universal steganography (that is
to say, steganography with maximum information embedding
rate, undetectable and only relying on the empirical distribution
of the host) for histogrambased hidden information detectors.
In the second scenario, permutation codes have been shown to
implement minimumdistortion perfect counterforensics (that is
to say, forgeries which are undetectable and as close as possible to
a target forgery) for histogrambased forensic detectors. Interestingly,
both of these developments have revealed connections with
compression through theoretical bounds from the mathematical
theory of information. In steganography, the longacknowledged
duality between perfect steganography and lossless compression
has been made explicit by permutation coding. On the other
hand, a connection between counterforensics, lossy compression
and histogram specification is also shown.
13th Workshop on Information Optics (WIO2014),Neuchâtel, Switzerland, 711 July, 2014
20140701T00:00:00ZAtypical Alstrom syndrome with novel ALMS1 mutations precluded by current diagnostic criteriaCasey, JillianMcGettigan, Paul A.Brosnahan, Donalet al.http://hdl.handle.net/10197/568920140704T15:11:42Z20140201T00:00:00ZAtypical Alstrom syndrome with novel ALMS1 mutations precluded by current diagnostic criteria
Casey, Jillian; McGettigan, Paul A.; Brosnahan, Donal; et al.
We report on clinical and genetic studies in a nonconsanguineous Irish sibpair with infantile dilated cardiomyopathy and retinopathy. A diagnosis of Alström Syndrome (AS) was considered and diagnostic testing pursued. The Alströms gene (ALMS1) is very large (23 exons) and diagnostic testing of mutational hotspots (exon 6, 8 and 10) was negative. Furthermore the siblings were tall and did not have the typical phenotype of nystagmus, photophobia, obesity or hearing loss and so the AS diagnosis was removed. We then sought to identify the causative gene in this family using whole exome sequencing. Unexpectedly, the exome analysis identified novel compound heterozygous ALMS1 mutations in exon 5 (c.777delT:p.D260fs*26) and exon 20 (c.12145_12146insC:p.S4049fs*36) that segregated with the phenotype. Although the siblings show some clinical overlap with AS, their phenotype is not classical. It is plausible that their atypical presentation may be due to the location of the ALMS1 mutations outside the usual mutational hotspots. Our findings show how atypical cases of AS may be missed under the current diagnostic guidelines and support consideration of complete ALMS1 sequencing in children with two or more features, even if all of the core clinical features of AS are not present.
20140201T00:00:00ZUnexpected genetic heterogeneity for primary ciliary dyskinesia in the Irish Traveller populationCasey, JillianMcGettigan, Paul A.Healy, FionaReynolds, AlisonKennedy, BreandánEnnis, SeanSlattery, DubhfeasaLynch, SallyHogg, Clairehttp://hdl.handle.net/10197/568820140704T13:55:28Z20140501T00:00:00ZUnexpected genetic heterogeneity for primary ciliary dyskinesia in the Irish Traveller population
Casey, Jillian; McGettigan, Paul A.; Healy, Fiona; Reynolds, Alison; Kennedy, Breandán; Ennis, Sean; Slattery, Dubhfeasa; Lynch, Sally; Hogg, Claire
We present a study of five children from three unrelated Irish Traveller families presenting with primary ciliary dyskinesia (PCD). As previously characterized disorders in the Irish Traveller population are caused by common homozygous mutations, we hypothesised that all three PCD families shared the same recessive mutation. However, exome sequencing showed that there was no pathogenic homozygous mutation common to all families. This finding was supported by histology, which showed that each family has a different type of ciliary defect; transposition defect (family A), nude epithelium (family B) and absence of inner and outer dynein arms (family C). Therefore, each family was analysed independently using homozygosity mapping and exome sequencing. The affected siblings in family A share a novel 1 bp duplication in RSPH4A (NM_001161664.1:c.166dup; p.Arg56Profs*11), a radialspoke head protein involved in ciliary movement. In family B, we identified three candidate genes (CCNO, KCNN3 and CDKN1C), with a 5bp duplication in CCNO (NM_021147.3:c.258_262dup; p.Gln88Argfs*8) being the most likely cause of ciliary aplasia. This is the first study to implicate CCNO, a DNA repair gene reported to be involved in multiciliogenesis, in PCD. In family C, we identified a ~3.5kb deletion in DYX1C1, a neuronal migration gene previously associated with PCD. This is the first report of a disorder in the relatively small Irish Traveller population to be caused by >1 disease gene. Our study identified at least three different PCD genes in the Irish Traveller population, highlighting that one cannot always assume genetic homogeneity, even in small consanguineous populations.
20140501T00:00:00ZOrigin of spurious single forces in the course mechanism of volcanic seismicityDe Barros, LouisLokmer, IvanBean, Christopher J.http://hdl.handle.net/10197/568220140702T13:35:20Z20130715T00:00:00ZOrigin of spurious single forces in the course mechanism of volcanic seismicity
De Barros, Louis; Lokmer, Ivan; Bean, Christopher J.
Single forces are often observed in the source mechanism of volcanic seismicity. However, their underlying causative processes are still doubtful. The reliability of single force observations must be assessed, prior to analyzing them in terms of physical mechanisms. Using numerical examples, we show that source mislocation and velocity mismodeling lead to strong spurious single forces. Layering in the velocity model produces converted Swaves and source mislocations modify the wavefield at the free surface (mainly through converted S and surface waves). However, these waves can also be accurately reproduced in a homogeneous model by adding a vertical single force in the source mechanism, which mainly generates Swaves for large takeoff angles. Hence approximate velocity models can lead to the appearance of strong single forces in source inversions. We conclude that, in moment tensor inversion, while single forces can be used in some cases to accommodate mismodeling errors, they cannot be reliably used to infer physical processes.
20130715T00:00:00ZThe selective oxidation of glycerol over model Au/TiO2 catalysts  the influence of glycerol purity on conversion and product selectivitySullivan, James A.Burnham, Sarahhttp://hdl.handle.net/10197/567520140627T13:59:50Z20140101T00:00:00ZThe selective oxidation of glycerol over model Au/TiO2 catalysts  the influence of glycerol purity on conversion and product selectivity
Sullivan, James A.; Burnham, Sarah
The activity and selectivity of a model Au/TiO2 catalyst was studied in the selective oxidation of glycerol as a function of the purity of the glycerol source. A reasonable conversion was noted when reagent grade starting materials were used. When crude glycerol from a FAME production facility was used, the activity of the catalyst was severely compromised and the selectivity of the reaction changed. Several lowcost approaches to purifying the crude glycerol were attempted but none resulted in the formation of a glycerol substrate whose conversion under reaction conditions matched that of the pure reagent grade substrate.
20140101T00:00:00ZInvestigating the source characteristics of longperiod (LP) seismic events recorded on Piton de la Fournaise volcano, La RéunionZecevic, MeganDe Barros, LouisBean, Christopher J.O'Brien, G. S.Brenguier, Florenthttp://hdl.handle.net/10197/566420140623T14:21:27Z20130515T00:00:00ZInvestigating the source characteristics of longperiod (LP) seismic events recorded on Piton de la Fournaise volcano, La Réunion
Zecevic, Megan; De Barros, Louis; Bean, Christopher J.; O'Brien, G. S.; Brenguier, Florent
Magmatic and hydrothermal processes play a significant role in generating seismicity at active volcanoes. These signals can be recorded at the surface and can be used to obtain an insight into the volcano's internal dynamics. Long period (LP) events are of particular interest as they often accompany or precede volcanic eruptions, but they are still not well understood. Piton de la Fournaise volcano, La Réunion Island, is one of the most active volcanoes in the world however LP events are rarely recorded there. A seismic network of 20 broadband seismometers has been operational on Piton de la Fournaise volcano since November 2009. Between November 2009 and January 2011 the volcano erupted five times, but only 15 LP events were recorded. Three of these eruptions were preceded by LP events, and several LP events were recorded during an intrusive phase. A family of three repeating LP events exists within the dataset. In order to characterize these events we locate and perform moment tensor inversion on the LP family. The LP events are located within the summit crater at shallow depths (< 200 m below the surface). Inversions show that the source mechanism is best represented by a tensile crack with horizontal crack geometry. We also investigate the relationship between LP occurrence and eruptive characteristics (size of the eruption, deformation of the edifice, etc.), and we find that the events exist only during flank eruptions and can be generated by the activity of the hydrothermal system and/or by the deformation inside the crater.
20130515T00:00:00ZMoment tensor inversion for the source location and mechanism of long period (LP) seismic events from 2009 at Turrialba volcano, Costa RicaEyre, Thomas S.Bean, Christopher J.De Barros, LouisO'Brien, G. S.Martini, FrancescaLokmer, Ivanet al.http://hdl.handle.net/10197/566320140623T13:39:32Z20130515T00:00:00ZMoment tensor inversion for the source location and mechanism of long period (LP) seismic events from 2009 at Turrialba volcano, Costa Rica
Eyre, Thomas S.; Bean, Christopher J.; De Barros, Louis; O'Brien, G. S.; Martini, Francesca; Lokmer, Ivan; et al.
Longperiod (LP) seismic events were recorded during the temporary installation of a broadband seismic network of 13 stations from March to September 2009 on Turrialba volcano, Costa Rica. Over 6000 LPs were extracted using a modified STA/LTA method and a family consisting of 435 similar LP events has been identified. For the first time at Turrialba volcano, fullwaveform moment tensor inversion is performed to jointly determine the location and source mechanism of the events. The LPs in the family are likely to be caused by crack mechanisms dipping towards the southwest at angles of approximately 10 to 20°, located at shallow depths (< 800 m) below the active Southwest and Central craters. As the locations are so shallow, the most probable causes of crack mechanisms are hydrothermal fluids resonating within or 'pulsing' through a crack. The waveforms observed at the summit stations suggest a 'pulsing' mechanism, but source resonance with a
high degree of damping is also possible.
20130515T00:00:00ZAn irregular lattice method for elastic wave propagationO'Brien, G. S.Bean, Christopher J.http://hdl.handle.net/10197/566020140619T14:55:04Z20111001T00:00:00ZAn irregular lattice method for elastic wave propagation
O'Brien, G. S.; Bean, Christopher J.
Lattice methods are a class of numerical scheme which represent a medium as a connection of interacting nodes or particles. In the case of modelling seismic wave propagation, the interaction term is determined from Hooke′s Law including a bondbending term. This approach has been shown to model isotropic seismic wave propagation in an elastic or viscoelastic medium by selecting the appropriate underlying lattice structure. To predetermine the material constants, this methodology has been restricted to regular grids, hexagonal or square in 2D or cubic in 3D. Here, we present a method for isotropic elastic wave propagation where we can remove this lattice restriction. The methodology is outlined and a relationship between the elastic material properties and an irregular lattice geometry are derived. The numerical method is compared with an analytical solution for wave propagation in an infinite homogeneous body along with comparing the method with a numerical solution for a layered elastic medium. The dispersion properties of this method are derived from a plane wave analysis showing the scheme is more dispersive than a regular lattice method. Therefore, the computational costs of using an irregular lattice are higher. However, by removing the regular lattice structure the anisotropic nature of fracture propagation in such methods can be removed.
20111001T00:00:00ZImaging magma storage below Teide volcano (Tenerife) using scattered seismic wavefieldsDe Barros, LouisMartini, FrancescaBean, Christopher J.GarciaYeguas, A.Ibáñez, J.http://hdl.handle.net/10197/564820140623T13:36:53Z20121101T00:00:00ZImaging magma storage below Teide volcano (Tenerife) using scattered seismic wavefields
De Barros, Louis; Martini, Francesca; Bean, Christopher J.; GarciaYeguas, A.; Ibáñez, J.
Tenerife (Canary Islands, Spain) is a volcanic island dominated by the TeidePico Viejo complex, with a summit height of 3718 m. After renewed signs of activity starting in 2004, an active seismic experiment was performed in 2007 to derive a tomographic model and identify seismic anomalies possibly associated with the magmatic system. To complement the tomography, a double beamforming analysis is applied on two orthogonal 2D profiles crossing the island to look for evidence of the existence of a magma chamber. Numerical tests allow us to investigate the best measure of coherency between traces, and show that the correlation and nth root semblance methods give better results than the classical semblance. They also demonstrate that the technique is reliable for locating scattering structures at depth, even when the velocity model is imperfect. Applying this technique to the Tenerife data set, two main anomalies can be identified: one at approximately 7–9 km b.s.l. depth in the northern part of the island, and one shallower (1–4 km b.s.l.) beneath the main summit. These structures could be linked to the magmatic system, in good agreement with previous studies. The shallowest one may be the phonolitic storage area feeding the TeidePico Viejo complex, while the deepest structure may be related to the basaltic system.
20121101T00:00:00ZTranscriptional regulation of the human thromboxane A2 receptor gene by Wilms' tumor (WT)1 and hypermethylated in cancer (HIC) 1 in prostate and breast cancersKeating, Garret L.Reid, Helen M.Eivers, Sarah B.Mulvaney, Eamon P.Kinsella, B. Theresehttp://hdl.handle.net/10197/564620140618T14:11:56Z20140601T00:00:00ZTranscriptional regulation of the human thromboxane A2 receptor gene by Wilms' tumor (WT)1 and hypermethylated in cancer (HIC) 1 in prostate and breast cancers
Keating, Garret L.; Reid, Helen M.; Eivers, Sarah B.; Mulvaney, Eamon P.; Kinsella, B. Therese
The prostanoid thromboxane (TX) A2 plays a central role in hemostasis and is increasingly implicated in neoplastic disease, including prostate and breast cancers. In humans, TXA2 signals through the TPα and TPβ isoforms of the T prostanoid receptor, two structurally related receptors transcriptionally regulated by distinct promoters, Prm1 and Prm3, respectively, within the TP gene. Focusing on TPα, the current study investigated its expression and transcriptional regulation through Prm1 in prostate and breast cancers. Expression of TPα correlated with increasing prostate and breast tissue tumor grade while the TXA2 mimetic U46619 promoted both proliferation and migration of the respective prostate (PC3) and breast (MCF7 and MDAMD231) derivedcarcinoma cell lines. Through 5′ deletional and genetic reporter analyses, several functional upstream repressor regions (URRs) were identified within Prm1 in PC3, MCF7 and MDAMB231 cells while sitedirected mutagenesis identified the tumor suppressors Wilms' tumor (WT)1 and hypermethylated in cancer (HIC) 1 as the transacting factors regulating those repressor regions. Chromatin immunoprecipitation (ChIP) studies confirmed that WT1 binds in vivo to multiple GCenriched WT1 ciselements within the URRs of Prm1 in PC3, MCF7 and MDAMB231 cells. Furthermore, ChIP analyses established that HIC1 binds in vivo to the HIC1(b)ciselement within Prm1 in PC3 and MCF7 cells but not in the MDAMB231 carcinoma line. Collectively, these data establish that WT1 and HIC1, both tumor suppressors implicated in prostate and breast cancers, transcriptionally repress TPα expression and thereby provide a strong genetic basis for understanding the role of TXA2 in the progression of certain human cancers.
20140601T00:00:00ZUniversal Taylor series, conformal mappings and boundary behaviourGardiner, Stephen J.http://hdl.handle.net/10197/564520140618T14:08:50Z20131201T00:00:00ZUniversal Taylor series, conformal mappings and boundary behaviour
Gardiner, Stephen J.
A holomorphic function f on a simply connected domain Ω is said to possess a universal Taylor series about a point in Ω if the partial sums of that series approximate arbitrary polynomials on arbitrary compacta K outside Ω (provided only that K has connected complement). This paper shows that this property is not conformally invariant, and, in the case where Ω is the unit disc, that such functions have extreme angular
boundary behaviour.
20131201T00:00:00ZBoundary Behaviour of Universal Taylor Series on Multiply Connected DomainsGardiner, Stephen J.Manolaki, Myrtohttp://hdl.handle.net/10197/564420140610T14:09:22Z20140501T00:00:00ZBoundary Behaviour of Universal Taylor Series on Multiply Connected Domains
Gardiner, Stephen J.; Manolaki, Myrto
A holomorphic function on a planar domain Ω is said to possess a universal Taylor series about a point ζ of Ω if subsequences of the partial sums of the Taylor series approximate arbitrary polynomials on arbitrary compact sets in C∖Ω that have connected complement. In the case where Ω is simply connected, such functions are known to be unbounded and to form a collection that is independent of the choice of ζ. This paper uses tools from potential theory to show that, even for domains Ω of arbitrary connectivity, such functions are unbounded whenever they exist. In the doubly connected case, a further analysis of boundary behaviour reveals that the collection of such functions can depend on the choice of ζ. This phenomenon was previously known only for domains that are at least triply connected. Related results are also established for universal Laurent series.
20140501T00:00:00ZStationary Boundary Points for a Laplacian Growth Problem in Higher DimensionsGardiner, Stephen J.Sjödin, Tomashttp://hdl.handle.net/10197/564320140610T14:07:31Z20140801T00:00:00ZStationary Boundary Points for a Laplacian Growth Problem in Higher Dimensions
Gardiner, Stephen J.; Sjödin, Tomas
It is known that corners of interior angle less than π/2 in the boundary of a plane domain are initially stationary for Hele–Shaw flow arising from an arbitrary injection point inside the domain. This paper establishes the corresponding result for Laplacian growth of domains in higher dimensions. The problem is treated in terms of evolving families of quadrature domains for subharmonic functions.
20140801T00:00:00ZRoutes to visible light active Cdoped TiO2 photocatalysts using carbon atoms from the Ti precursorsSullivan, James A.Neville, Elaine M.Herron, RoryThampi, RavinandrathanMacElroy, J. M. Donhttp://hdl.handle.net/10197/564020140610T13:56:51Z20140529T00:00:00ZRoutes to visible light active Cdoped TiO2 photocatalysts using carbon atoms from the Ti precursors
Sullivan, James A.; Neville, Elaine M.; Herron, Rory; Thampi, Ravinandrathan; MacElroy, J. M. Don
In this work, different thermal treatments of titanium isopropoxidederived photocatalyst precursors, designed with the purpose of generating Cdoped TiO2 photocatalysts using carbon atoms present in the TiOx gel precursors as dopants, are presented. Specifically, these look at varying the standard calcination techniques using heat treatments in He (rather than calcination in air) and lower temperature calcinations (200 °C rather than 500 °C). The formed materials are characterised using N2 physisorption, XRD, UV Visible spectroscopy and XPS and their activities in promoting the oxidation of 4chlorophenol under visiblelightonly conditions were analysed. The nature of carbon remaining on the (or in the) material is discussed found to be both surface graphitic carbon and carbon present in anionic dopant positions. The different contributions of each type of carbon to the catalytic photoactivity under visible light are discussed.
20140529T00:00:00ZFilamentous fungal biofilm for production of human drug metabolitesAmadio, JessicaCasey, EoinMurphy, Cormac D.http://hdl.handle.net/10197/563520140609T14:11:42Z20130701T00:00:00ZFilamentous fungal biofilm for production of human drug metabolites
Amadio, Jessica; Casey, Eoin; Murphy, Cormac D.
In drug development, access to drug metabolites is essential for assessment of toxicity and pharmacokinetic studies. Metabolites are usually acquired via chemical synthesis, although biological production is potentially more efficient with fewer waste management issues. A significant problem with the biological approach is the effective halflife of the biocatalyst, which can be resolved by immobilisation. The fungus Cunninghamella elegans is well established as a model of mammalian metabolism, although it has not yet been used to produce metabolites on a large scale. Here, we describe immobilisation of C. elegans as a biofilm, which can transform drugs to important human metabolites. The biofilm was cultivated on hydrophilic microtiter plates and in shake flasks containing a steel spring in contact with the glass. Fluorescence and confocal scanning laser microscopy revealed that the biofilm was composed of a dense network of hyphae, and biochemical analysis demonstrated that the matrix was predominantly polysaccharide. The medium composition was crucial for both biofilm formation and biotransformation of flurbiprofen. In shake flasks, the biofilm transformed 86% of the flurbiprofen added to hydroxylated metabolites within 24 h, which was slightly more than planktonic cultures (76%). The biofilm had a longer effective lifetime than the planktonic cells, which underwent lysis after 2×72 h cycles, and diluting the Sabouraud dextrose broth enabled the thickness of the biofilm to be controlled while retaining transformation efficiency. Thus, C. elegans biofilm has the potential to be applied as a robust biocatalyst for the production of human drug metabolites required for drug development.
20130701T00:00:00ZHemiacetal stabilization in a chymotrypsin inhibitor complex and the reactivity of the hydroxyl group of the catalytic serine residue of chymotrypsinCleary, Jennifer A.Doherty, WilliamEvans, PaulMalthouse, J.Paul G.http://hdl.handle.net/10197/562920140604T13:53:52Z20140601T00:00:00ZHemiacetal stabilization in a chymotrypsin inhibitor complex and the reactivity of the hydroxyl group of the catalytic serine residue of chymotrypsin
Cleary, Jennifer A.; Doherty, William; Evans, Paul; Malthouse, J.Paul G.
The aldehyde inhibitor ZAlaAlaPheCHO has been synthesized and shown by 13CNMR to react with the active site serine hydroxyl group of alphachymotrypsin to form two diastereomeric hemiacetals. For both hemiacetals oxyanion formation occurs with a pKa value of ~ 7 showing that chymotrypsin reduces the oxyanion pKa values by ~ 5.6 pKa units and stabilizes the oxyanions of both diastereoisomers by ~ 32 kJ mol− 1. As pH has only a small effect on binding we conclude that oxyanion formation does not have a significant effect on binding the aldehyde inhibitor. By comparing the binding of ZAlaAlaPheCHO with that of ZAlaAlaPheH we estimate that the aldehyde group increases binding ~ 100 fold. At pH 7.2 the effective molarity of the active site serine hydroxy group is ~ 6000 which is ~ 7 × less effective than with the corresponding glyoxal inhibitor. Using 1HNMR we have shown that at both 4 and 25 °C the histidine pKa is ~ 7.3 in free chymotrypsin and it is raised to ~ 8 when ZAlaAlaPheCHO is bound. We conclude that oxyanion formation only has a minor role in raising the histidine pKa and that the aldehyde hydrogen must be replaced by a larger group to raise the histidine pKa > 10 and give stereospecific formation of tetrahedral intermediates. The results show that a large increase in the pKa of the active site histidine is not needed for the active site serine hydroxyl group to have an effective molarity of 6000.
20140601T00:00:00ZA role for prolyl hydroxylase domain proteins in hippocampal synaptic plasticityCorcoran, AlanKunze, ReinerHarney, Sarah C.O'Connor, J. J.et al.http://hdl.handle.net/10197/562420140606T03:00:08Z20131001T00:00:00ZA role for prolyl hydroxylase domain proteins in hippocampal synaptic plasticity
Corcoran, Alan; Kunze, Reiner; Harney, Sarah C.; O'Connor, J. J.; et al.
Hypoxiainducible factors (HIFs) are key transcriptional regulators that play a major role in oxygen homeostasis. HIF activity is tightly regulated by oxygendependent hydroxylases, which additionally require iron and 2oxoglutarate as cofactors. Inhibition of these enzymes has become a novel target to modulate the hypoxic response for therapeutic benefit. Inhibition of prolyl4hydroxylase domains (PHDs) have been shown to delay neuronal cell death and protect against ischemic injury in the hippocampus. In this study we have examined the effects of prolyl hydroxylase inhibition on synaptic transmission and plasticity in the hippocampus. Field excitatory postsynaptic potentials (fEPSPs) and excitatory postsynaptic currents (EPSCs) were elicited by stimulation of the Schaffer collateral pathway in the CA1 region of the hippocampus. Treatment of rat hippocampal slices with low concentrations (10 µM) of the iron chelator deferosoxamine (DFO) or the 2oxoglutarate analogue dimethyloxalyl glycine (DMOG) had no effect on fEPSP. In contrast, application of 1 mM DMOG resulted in a significant decrease in fEPSP slope. Antagonism of the NMDA receptor attenuated the effects of DMOG on baseline synaptic signalling. In rat hippocampal slices pretreated with DMOG and DFO the induction of longterm potentiation (LTP) by tetanic stimulation was strongly impaired. Similarly, neuronal knockout of the single PHD family member PHD2 prevented murine hippocampal LTP. Preconditioning of PHD2 deficient hippocampi with either DMOG, DFO, or the PHD specific inhibitor JNJ42041935, did not further decrease LTP suggesting that DMOG and DFO influences synaptic plasticity primarily by inhibiting PHDs rather than unspecific effects. These findings provide striking evidence for a modulatory role of PHD proteins on synaptic plasticity in the hippocampus.
20131001T00:00:00ZThe role of permutation coding in minimumdistortion perfect counterforensicsBalado, Félixhttp://hdl.handle.net/10197/556520140509T03:00:07Z20140501T00:00:00ZThe role of permutation coding in minimumdistortion perfect counterforensics
Balado, Félix
This paper exploits the connection between minimumdistortion perfect counterforensics and maximumrate perfect steganography in order to provide the optimum solution to the first of these problems, in the case in which the forensic detector solely uses first order statistics. The solution relies on Slepian’s variant I permutation codes, which had previously been shown to implement maximum
rate perfect steganography when the host is memoryless (equivalently, when the steganographic detector only uses firstorder statistics). Additionally, we demonstrate a blind counterforensic strategy made possible by permutation decoding, which may also find application in image processing.
39th IEEE International Conference on Acoustics, Speech and Signal Processing (ICASSP), May, 2014
20140501T00:00:00ZDual harmonic Kelvin probe force microscopy at the grapheneliquid interfaceCollins, LiamKilpatrick, J. I.Vlassiouk, Ivan V.Weber, Stefan A. L.Rodriguez, Brian J.et al.http://hdl.handle.net/10197/556420140428T14:00:21Z20140401T00:00:00ZDual harmonic Kelvin probe force microscopy at the grapheneliquid interface
Collins, Liam; Kilpatrick, J. I.; Vlassiouk, Ivan V.; Weber, Stefan A. L.; Rodriguez, Brian J.; et al.
Kelvin probe force microscopy (KPFM) is a powerful technique for the determination of the contact potential difference (CPD) between an atomic force microscope tip and a sample under ambient and vacuum conditions. However, for many energy storage and conversion systems, including graphenebased electrochemical capacitors, understanding electrochemical phenomena at the solid¿liquid interface is paramount. Despite the vast potential to provide fundamental insight for energy storage materials at the nanoscale, KPFM has found limited applicability in liquid environments to date. Here, using dual harmonic (DH)KPFM, we demonstrate CPD imaging of graphene in liquid. We find good agreement with measurements performed in air, highlighting the potential of DHKPFM to probe electrochemistry at the graphene¿liquid interface.
20140401T00:00:00ZA convenient chemicalmicrobial method for developing fluorinated pharmaceuticalsBright, Tara V.Dalton, FayElder, Victoria L.Murphy, Cormac D.O'Connor, Neil K.Sandford, Grahamhttp://hdl.handle.net/10197/555720140423T14:12:35Z20130101T00:00:00ZA convenient chemicalmicrobial method for developing fluorinated pharmaceuticals
Bright, Tara V.; Dalton, Fay; Elder, Victoria L.; Murphy, Cormac D.; O'Connor, Neil K.; Sandford, Graham
A significant proportion of pharmaceuticals are fluorinated and selecting the site of fluorine incorporation can be an important beneficial part a drug development process. Here we describe initial experiments aimed at the development of a general method
of selecting optimum sites on pro  drug molecules for fluorination, so that metabolic stability may be improved. Several model biphenyl derivatives were transformed by the fungus Cunninghamella elegans and the bacterium Streptomyces griseus, both of which contain cytochromes P450 that mimic oxidation processes in vivo, so that the site of oxidation could be determined. Subsequently, fluorinated biphenyl derivatives were synthesised using appropriate Suzuki  Miyaura coupling reactions, positioning the fluorine atom at the pre  determined site of microbial oxidation; the fluorinated biphenyl derivatives were incubated with the microorganisms and the degree of oxidation assessed. Biphenyl4carboxylic acid was transformed completely to 4'  hydroxybiphenyl  4  carboxylic acid by C. elegans but, in contrast, the 4' fluoro  analogue remained untransformed exemplifying the microbial oxidation – chemical fluorination concept. 2' 
Fluoroand 3'  fluoro  biphenyl  4  carboxylic acid were also transformed, but more slowly than the non  fluorinated biphenyl carboxylic acid derivative. Thus, it is possible to design compounds in an iterative fashion with a longer metabolic half  life by identifying the sites that are most easily oxidised by in vitro methods and subsequent fluorination without recourse to extensive animal studies.
20130101T00:00:00ZPolyharmonic functions of infinite order on annular regionsKounchev, OgnyanRender, Hermannhttp://hdl.handle.net/10197/555620140423T14:06:47Z20130601T00:00:00ZPolyharmonic functions of infinite order on annular regions
Kounchev, Ognyan; Render, Hermann
Polyharmonic functions f of in nite order and type on annular regions are
systematically studied. The rst main result states that the FourierLaplace coefficients
fk;l (r) of a polyharmonic function f of in nite order and type 0 can be extended to
analytic functions on the complex plane cut along the negative semiaxis. The second
main result gives a constructive procedure via FourierLaplace series for the analytic
extension of a polyharmonic function on annular region A(r0; r1) of in nite order and
type less than 1=2r1 to the kernel of the harmonicity hull of the annular region. The
methods of proof depend on an extensive investigation of Taylor series with respect to
linear differential operators with constant coefficients.
20130601T00:00:00ZThe approximation order of polysplinesKounchev, OgnyanRender, Hermannhttp://hdl.handle.net/10197/551920140404T11:41:40Z20040701T00:00:00ZThe approximation order of polysplines
Kounchev, Ognyan; Render, Hermann
We show that the scaling spaces de ned by the polysplines of order
p provide approximation order 2p: For that purpose we re ne the re
sults on one dimensional approximation order by Lsplines obtained
in [2].
20040701T00:00:00ZVector piezoresponse force microscopyKalinin, S. V.Rodriguez, Brian J.Jesse, S.et al.http://hdl.handle.net/10197/551420140404T10:44:39Z20060601T00:00:00ZVector piezoresponse force microscopy
Kalinin, S. V.; Rodriguez, Brian J.; Jesse, S.; et al.
A novel approach for nanoscale imaging and characterization of the orientation dependence of electromechanical propertiesvector piezoresponse force microscopy (Vector PFM)is described. The relationship between local electromechanical response, polarization, piezoelectric constants, and crystallographic orientation is analyzed in detail. The image formation mechanism in vector PFM is discussed. Conditions for complete threedimensional (313) reconstruction of the electromechanical response vector and evaluation of the piezoelectric constants from PFM data are set forth. The developed approach can be applied to crystallographic orientation imaging in piezoelectric materials with a spatial resolution below 10 nm. Several approaches for data representation in 2DPFM and 3DPFM are presented. The potential of vector PFM for molecular orientation imaging in macroscopically disordered piezoelectric polymers and biological systems is discussed.
20060601T00:00:00ZPolyharmonicity and algebraic support of measuresKounchev, OgnyanRender, Hermannhttp://hdl.handle.net/10197/551120140401T08:34:23Z20070201T00:00:00ZPolyharmonicity and algebraic support of measures
Kounchev, Ognyan; Render, Hermann
Our main result states that two signed measures μ and ν with bounded
support contained in the zero set of a polynomial P(χ) are equal if they coincide on the
subspace of all polynomials of polyharmonic degree NP where the natural number NP is
explicitly computed by the properties of the polynomial P(χ). The method of proof
depends on a definition of a multivariate Markov transform which is another major
objective of the present paper. The classical notion of orthogonal polynomial of second
kind is generalized to the multivariate setting: it is a polyharmonic function which has
similar features to those in the onedimensional case.
20070201T00:00:00ZConvergence of polyharmonic splines on semiregular grids Z x aZ^n for a to 0Kounchev, OgnyanRender, Hermannhttp://hdl.handle.net/10197/551020140401T08:31:20Z20070701T00:00:00ZConvergence of polyharmonic splines on semiregular grids Z x aZ^n for a to 0
Kounchev, Ognyan; Render, Hermann
Let
p,n
∈
N
with 2
p
≥
n
+ 2
,
and let
I
a
be a polyharmonic spline of
order
p
on the grid
Z
×
a
Z
n
which satisfies the interpolating conditions
I
a
(
j,am
) =
d
j
(
am
) for
j
∈
Z
,m
∈
Z
n
where the functions
d
j
:
R
n
→
R
and the parameter
a>
0 are given. Let
B
s
(
R
n
) be the set of all integrable
functions
f
:
R
n
→
C
such that the integral
k
f
k
s
:=
Z
R
n
b
f
(
ξ
)
(1 +

ξ

s
)
dξ
is finite. The main result states that for given
σ
≥
0 there exists a
constant
c>
0 such that whenever
d
j
∈
B
2
p
(
R
n
)
∩
C
(
R
n
)
,j
∈
Z
,
satisfy
k
d
j
k
2
p
≤
D
·
(1 +

j

σ
) for all
j
∈
Z
there exists a polyspline
S
:
R
n
+1
→
C
of order
p
on strips such that

S
(
t,y
)
−
I
a
(
t,y
)
≤
a
2
p
−
1
c
·
D
·
(1 +

t

σ
)
for all
y
∈
R
n
,t
∈
R
and all 0
<a
≤
1.
20070701T00:00:00ZOn realanalytic recurrence relations for cardinal exponential BsplinesAldaz, J. M.Kounchev, OgnyanRender, Hermannhttp://hdl.handle.net/10197/550820140401T08:23:56Z20071001T00:00:00ZOn realanalytic recurrence relations for cardinal exponential Bsplines
Aldaz, J. M.; Kounchev, Ognyan; Render, Hermann
Let LN+1 be a linear differential operator of order N + 1 with constant coefficients
and real eigenvalues λ 1, ..., λ N+1, let E( N+1) be the space of all C∞solutions of
LN+1 on the real line.We show that for N 2 and n = 2, ...,N, there is a recurrence
relation from suitable subspaces εn to εn+1 involving realanalytic functions, and
with εN+1 = E(Λ N+1) if and only if contiguous eigenvalues are equally spaced.
20071001T00:00:00ZPadé approximation for a multivariate Markov transformKounchev, OgnyanRender, Hermannhttp://hdl.handle.net/10197/550320140331T09:18:05Z20081001T00:00:00ZPadé approximation for a multivariate Markov transform
Kounchev, Ognyan; Render, Hermann
Methods of Padè approximation are used to analyse a multivariate
Markov transform which has been recently introduced by the authors.
The first main result is a characterization of the rationality of the
Markov transform via Hankel determinants. The second main result
is a cubature formula for a special class of measure.
20081001T00:00:00ZOn the mixed Cauchy problem with data on singular conicsEbenfelt, PeterRender, Hermannhttp://hdl.handle.net/10197/550120140328T09:37:53Z20080801T00:00:00ZOn the mixed Cauchy problem with data on singular conics
Ebenfelt, Peter; Render, Hermann
We consider a problem of mixed Cauchy type for certain holomorphic partial differential
operators with the principal part Q2p(D) essentially being the (complex) Laplace operator to
a power, Δp. We provide inital data on a singular conic divisor given by P = 0, where P is a
homogeneous polynomial of degree 2p. We show that this problem is uniquely solvable if the
polynomial P is elliptic, in a certain sense, with respect to the principal part Q2p(D).
20080801T00:00:00ZThe Goursat problem for a generalized Helmholtz operator in the planeEbenfelt, PeterRender, Hermannhttp://hdl.handle.net/10197/550020140328T09:34:45Z20080901T00:00:00ZThe Goursat problem for a generalized Helmholtz operator in the plane
Ebenfelt, Peter; Render, Hermann
We consider the Goursat problem in the plane for partial differential operators whose principal part is the pth power of the standard Laplace operator. The data is posed on a union of 2p distinct lines through the origin. We show that the solvability of this Goursat problem depends on Diophantine properties of the geometry of lines on which the data is posed.
20080901T00:00:00ZReproducing kernels for polyharmonic polynomialsRender, Hermannhttp://hdl.handle.net/10197/549920140328T09:31:08Z20081001T00:00:00ZReproducing kernels for polyharmonic polynomials
Render, Hermann
The reproducing kernel of the space of all homogeneous polynomi
als of degree
k
and polyharmonic order
m
is computed explicitly, solving a
question of A. Fryant and M.K. Vemuri.
20081001T00:00:00ZBernstein operators for exponential polynomialsAldaz, J. M.Kounchev, OgnyanRender, Hermannhttp://hdl.handle.net/10197/549820140328T09:30:16Z20090401T00:00:00ZBernstein operators for exponential polynomials
Aldaz, J. M.; Kounchev, Ognyan; Render, Hermann
Let
L
be a linear differential operator with constant coefficients of order
n
and complex eigenvalues
λ
0
,...,λ
n
. Assume that the set
U
n
of all solutions of the
equation
Lf
= 0 is closed under complex conjugation. If the length of the interval [
a,b
]
is smaller than
π/M
n
, where
M
n
:= max
{
Im
λ
j

:
j
= 0
,...,n
}
, then there exists a basis
p
n,k
,
k
= 0
,...n
, of the space
U
n
with the property that each
p
n,k
has a zero of order
k
at
a
and a zero of order
n
−
k
at
b,
and each
p
n,k
is positive on the open interval (
a,b
)
.
Under the additional assumption that
λ
0
and
λ
1
are real and distinct, our first main
result states that there exist points
a
=
t
0
<t
1
<...<t
n
=
b
and positive numbers
α
0
,..,α
n
, such that the operator
B
n
f
:=
n
X
k
=0
α
k
f
(
t
k
)
p
n,k
(
x
)
satisfies
B
n
e
λ
j
x
=
e
λ
j
x
, for
j
= 0
,
1
.
The second main result gives a sufficient condition
guaranteeing the uniform convergence of
B
n
f
to
f
for each
f
∈
C
[
a,b
].
20090401T00:00:00ZOn the Bernstein operator of S. Morigi and M. NeamtuKounchev, OgnyanRender, Hermannhttp://hdl.handle.net/10197/549620140328T09:07:13Z20091001T00:00:00ZOn the Bernstein operator of S. Morigi and M. Neamtu
Kounchev, Ognyan; Render, Hermann
We discuss a Bernstein type operator introduced by S. Morigi and
M. Neamtu for
D
polynomials in the more general framework of exponential
polynomials
20091001T00:00:00ZShape preserving properties of generalized Bernstein operators on extended Chebyshev spacesAldaz, J. M.Kounchev, OgnyanRender, Hermannhttp://hdl.handle.net/10197/549520140328T09:05:29Z20091201T00:00:00ZShape preserving properties of generalized Bernstein operators on extended Chebyshev spaces
Aldaz, J. M.; Kounchev, Ognyan; Render, Hermann
We study the existence and shape preserving properties of a generalized
Bernstein operator
B
n
fixing a strictly positive function
f
0
, and a second function
f
1
such
that
f
1
/f
0
is strictly increasing, within the framework of extended Chebyshev spaces
U
n
.
The first main result gives an inductive criterion for existence: suppose there exists a
Bernstein operator
B
n
:
C
[
a,b
]
→
U
n
with strictly increasing nodes, fixing
f
0
,f
1
∈
U
n
.
If
U
n
⊂
U
n
+1
and
U
n
+1
has a nonnegative Bernstein basis, then there exists a Bernstein
operator
B
n
+1
:
C
[
a,b
]
→
U
n
+1
with strictly increasing nodes, fixing
f
0
and
f
1
.
In
particular, if
f
0
,f
1
,...,f
n
is a basis of
U
n
such that the linear span of
f
0
,..,f
k
is an
extended Chebyshev space over [
a,b
] for each
k
= 0
,...,n
, then there exists a Bernstein
operator
B
n
with increasing nodes fixing
f
0
and
f
1
.
The second main result says that
under the above assumptions the following inequalities hold
B
n
f
≥
B
n
+1
f
≥
f
for all (
f
0
,f
1
)convex functions
f
∈
C
[
a,b
]
.
Furthermore,
B
n
f
is (
f
0
,f
1
)convex for all
(
f
0
,f
1
)convex functions
f
∈
C
[
a,b
]
.
20091201T00:00:00ZCauchy, Goursat and Dirichlet problems for holomorphic partial differential equationsRender, Hermannhttp://hdl.handle.net/10197/549220140327T15:57:12Z20110101T00:00:00ZCauchy, Goursat and Dirichlet problems for holomorphic partial differential equations
Render, Hermann
n this paper we survey recent results about Fischer decomposi
tions of polynomials or entire functions and their applications to holomorphic
partial di erential equations. We discuss Cauchy and Goursat problems for the
polyharmonic operator. Special emphasis is given to the KhavinsonShapiro
conjecture concerning polynomial solvability of the Dirichlet problem.
20110101T00:00:00ZThe KhavinsonShapiro conjecture and polynomial decompositionsLundberg, ErikRender, Hermannhttp://hdl.handle.net/10197/549020140327T15:51:19Z20110415T00:00:00ZThe KhavinsonShapiro conjecture and polynomial decompositions
Lundberg, Erik; Render, Hermann
The main result of the paper states the following: Let ψ be a polynomial
in n variables of degree t: Suppose that there exists a constant C > 0 such
that any polynomial f has a polynomial decomposition f = ψ qf + hf with
khf = 0 and deg qf deg f + C: Then deg ψ 2k. Here ∆k is the kth
iterate of the Laplace operator ∆ : As an application, new classes of domains
in Rn are identi ed for which the KhavinsonShapiro conjecture holds.
20110415T00:00:00ZPolyharmonic Hardy spaces on the complexified annulus and error estimates of cubature formulasKounchev, OgnyanRender, Hermannhttp://hdl.handle.net/10197/548920140327T15:50:00Z20121201T00:00:00ZPolyharmonic Hardy spaces on the complexified annulus and error estimates of cubature formulas
Kounchev, Ognyan; Render, Hermann
The present paper has a twofold contribution: first, we intro
duce a new concept of Hardy spaces on a multidimensional complexified
annular domain which is closely related to the annulus of the KleinDi
rac
quadric important in Conformal Quantum Field Theory. Secondly, for
functions in these Hardy spaces, we provide error estimate for the p
oly
harmonic GaußJacobi cubature formulas, which have been introduced
in previous papers.
20121201T00:00:00ZHarmonic divisors and rationality of zeros of Jacobi polynomialsRender, Hermannhttp://hdl.handle.net/10197/548820140327T15:47:30Z20130801T00:00:00ZHarmonic divisors and rationality of zeros of Jacobi polynomials
Render, Hermann
Let
Pn
(α,β
)
(
x
)
be the Jacobi polynomial of degree
n
with parameters
αβ
The main result of the paper states the following: If
b≠
1
;
3
and
c
are nonzero rel
atively prime natural numbers then
P
(
k
+(
d
3)
=
2
;k
+(
d
3)
=
2)
n
p
b=c
6
≠ 0
for all natural
numbers
d;n
and
k
2
N
0
:
Moreover, under the above assumption, the polynomial
Q
(
x
) =
b
c
x
2
1
+
:::
+
x
2
d
1
+
b
c
1
x
2
d
is not a harmonic divisor, and the Dirichlet problem for
the cone
f
Q
(
x
)
<
0
g
has polynomial harmonic solutions for polynomial data functions.
20130801T00:00:00ZRegularity of generalized Daubechies wavelets reproducing exponential polynomials with realvalued parametersDyn, NiraKounchev, OgnyanLevin, DavidRender, Hermannhttp://hdl.handle.net/10197/548420140327T15:27:02Z20140102T00:00:00ZRegularity of generalized Daubechies wavelets reproducing exponential polynomials with realvalued parameters
Dyn, Nira; Kounchev, Ognyan; Levin, David; Render, Hermann
We investigate nonstationary orthogonal wavelets based on a nonstationary
interpolatory subdivision scheme reproducing a given set of exponentials with realvalued
parameters. The construction is analogous to the construction of Daubechies wavelets
using the subdivision scheme of DeslauriersDubuc. The main result is the existence and
smoothness of these Daubechies type wavelets.
20140102T00:00:00ZConvergence of rational Bernstein operatorsRender, Hermannhttp://hdl.handle.net/10197/547820140327T14:32:26Z20140401T00:00:00ZConvergence of rational Bernstein operators
Render, Hermann
In this paper we discuss convergence properties and error estimates of rational
Bernstein operators introduced by P. Pit¸ul and P. Sablonni`ere. It is shown that the
rational Bernstein operators converge to the identity operator if and only if the maximal
difference between two consecutive nodes is converging to zero. Further a Voronovskaja
theorem is given based on the explicit computation of higher order moments for the
rational Bernstein operator
20140401T00:00:00ZQuantitative determination of tip parameters in piezoresponse force microscopyKalinin, S. V.Jesse, S.Rodriguez, Brian J.et al.http://hdl.handle.net/10197/547520140320T16:00:25Z20070524T00:00:00ZQuantitative determination of tip parameters in piezoresponse force microscopy
Kalinin, S. V.; Jesse, S.; Rodriguez, Brian J.; et al.
One of the key limiting factors in the quantitative interpretation of piezoresponse force microscopy (PFM) is the lack of knowledge on the effective tip geometry. Here the authors derive analytical expressions for a 180 degrees domain wall profile in PFM for the point charge, sphere plane, and disk electrode models of the tip. An approach for the determination of the effective tip parameters from the wall profile is suggested and illustrated for several ferroelectric materials. The calculated tip parameters can be used selfconsistently for the interpretation of PFM resolution and spectroscopy data, i.e., linear imaging processes.
20070524T00:00:00ZReal Bargmann spaces, Fischer decompositions and Sets of uniqueness for polyharmonic functionsRender, Hermannhttp://hdl.handle.net/10197/547420140320T15:48:50Z20080401T00:00:00ZReal Bargmann spaces, Fischer decompositions and Sets of uniqueness for polyharmonic functions
Render, Hermann
In this paper a positive answer is given to the following question of W.K.
Hayman: if a polyharmonic entire function of order k vanishes on k distinct ellipsoids
in the euclidean space Rn then it vanishes everywhere. Moreover a characterization of
ellipsoids is given in terms of an extension property of solutions of entire data functions
for the Dirichlet problem answering a question of D. Khavinson and H.S. Shapiro. These
results are consequences from a more general result in the context of direct sum decompositions
(Fischer decompositions) of polynomials or functions in the algebra A(BR)
of all realanalytic functions defined on the ball BR of radius R and center 0 whose
Taylor series of homogeneous polynomials converges compactly in BR. The main result
states that for a given elliptic polynomial P of degree 2k and sufficiently large radius
R > 0 the following decomposition holds: for each function f 2 A(BR) there exist
unique q, r 2 A(BR) such that f = Pq + r and kr = 0. Another application of this
result is the existence of polynomial solutions of the polyharmonic equation ku = 0 for
polynomial data on certain classes of algebraic hypersurfaces.
2000 Mathematical Subject Classification. Primary: 31B30. Secondary: 35A20,
14P99, 12Y05
20080401T00:00:00ZCoseismic velocity variations caused by static stress changes associated with the 2001 Mw=4.3 Agios Ionis earthquake in the Gulf of Corinth, GreeceCociani, L.Bean, Christopher J.LyonCaen, Heleneet al.http://hdl.handle.net/10197/544320140228T10:01:56Z20100701T00:00:00ZCoseismic velocity variations caused by static stress changes associated with the 2001 Mw=4.3 Agios Ionis earthquake in the Gulf of Corinth, Greece
Cociani, L.; Bean, Christopher J.; LyonCaen, Helene; et al.
The analysis of temporal variations in the seismic velocity across faults can be used to
estimate in situ stress changes. Seismic velocity of propagation depends on the fault
stiffness, which is a function of stress. The coda wave interferometry technique is applied
to seven families of repeating earthquakes (multiplets) recorded on the southern shore of
the Gulf of Corinth, Greece, to estimate high precision velocity changes in the Earth
’
s
crust associated with the
M
w
= 4.3 Agios Ioanis earthquake. Results show that the Agios
Ioanis event causes a perturbation in elastic properties at seismogenic depth, resulting in a
reduction of 0.2% in the seismic velocity. The results are not consistent with either damage
induced by dynamic stresses nor a fluid transient origin. In contrast, both the spatial
distribution and magnitude of the velocity perturbation correlate well with modeled static
stress variations. This suggests that the measured changes in the mechanical properties of
the seismogenic crust can be attributed to a change in static stress field associated with the
M
w
= 4.3 Agios Ioanis earthquake. The velocity changes indicate an unclamping of the
Pyrgaki fault at depth, which has local hazard implications
20100701T00:00:00ZStatistical selection of the 'best' seismic source mechanisms from inversions of synthetic volcanic longperiod eventsO'Brien, G. S.Lokmer, IvanBean, Christopher J.http://hdl.handle.net/10197/544120140228T09:51:23Z20100901T00:00:00ZStatistical selection of the 'best' seismic source mechanisms from inversions of synthetic volcanic longperiod events
O'Brien, G. S.; Lokmer, Ivan; Bean, Christopher J.
Inversions for the source mechanism of long‐period (LP) seismicity recorded on
volcanoes have become increasingly common and are used to interpret fluid‐driven
processes. The source mechanism considered for LP inversions usually consists of a
symmetric moment tensor with and without single forces. Also, constrained inversions have
been performed where one presumes a specific source geometry that reduces the degrees of
freedom in the inversion. To select the correct solution from the different possible
mechanisms, the Akaike information criterion (AIC) has been used. However, since
AIC performs well only if the inverted model is close to the true model, we tested its
ability to select the correct model in LP inversions. Using synthetic data sets generated on
Mt. Etna, Italy, with a tomography velocity model and the Green’s functions calculated for
a simplified, homogeneous velocity model, we have investigated (1) if any of the inversion
source models can recover the true mechanism and (2) the ability of the Akaike and
Bayesian information criteria (BIC) to select the correct model. Results show that in some
cases it is possible to recover the mechanism but never the source magnitude and that the
BIC is a better measure than the AIC in selecting the true source model, although in
numerous cases both criteria fail to select the correct solution. Therefore, the BIC should
be used as opposed to the AIC if it is necessary to select an appropriate source. Caution
should be used when using the statistical measure in any seismic inversion application.
20100901T00:00:00ZSource mechanism of long period events recorded by a high density seismic network during the 2008 eruption on Mount EtnaDe Barros, LouisLokmer, IvanBean, Christopher J.O'Brien, G. S.Zuccarello, Lucianoet al.http://hdl.handle.net/10197/544020140228T09:46:25Z20110101T00:00:00ZSource mechanism of long period events recorded by a high density seismic network during the 2008 eruption on Mount Etna
De Barros, Louis; Lokmer, Ivan; Bean, Christopher J.; O'Brien, G. S.; Zuccarello, Luciano; et al.
129 Long Period (LP) events, divided into two families of similar
events, were recorded by the 50 stations deployed on Mount Etna in the
second half of June 2008. During this period lava was flowing from a
lateral fracture after a summit strombolian eruption. In order to
understand the mechanisms of these events, we perform moment tensor
inversions. Inversions are initially kept unconstrained to estimate the
most likely mechanism. Numerical tests show that unconstrained inversion
leads to reliable moment tensor solutions because of the close
proximity of numerous stations to the source positions. However, single
forces cannot be accurately determined as they are very sensitive to
uncertainties in the velocity model. Constrained inversions for a crack,
a pipe or an explosion then allow us to accurately determine the
structural orientations of the source mechanisms. Both numerical tests
and LP event inversions emphasise the importance of using stations
located as close as possible to the source. Inversions for both
families show mechanisms with a strong volumetric component. These
events are most likely generated by cracks striking SWNE for both
families and dipping 70 degrees SE (Family 1) and 50 degrees NW
(Family 2). For Family 1 events, the crack geometry is nearly orthogonal
to the dikelike structure along which events are located, while for
Family 2 the location gave two pipelike bodies which belong to the same
plane as the crack mechanism. The orientations of the cracks are
consistent with local tectonics, which shows a SWNE weakness direction.
The LP events appear to be a response to the lava fountain occurring on
the 10th of May, 2008 as opposed to the flank lava flow.
20110101T00:00:00ZTime reverse location of seismic longperiod events recorded on Mt EtnaO'Brien, G. S.Lokmer, IvanDe Barros, LouisBean, Christopher J.Saccorotti, GilbertoMétaxian, J. P.Patané, Domenicohttp://hdl.handle.net/10197/543920140618T14:12:52Z20110101T00:00:00ZTime reverse location of seismic longperiod events recorded on Mt Etna
O'Brien, G. S.; Lokmer, Ivan; De Barros, Louis; Bean, Christopher J.; Saccorotti, Gilberto; Métaxian, J. P.; Patané, Domenico
We
present the first application of a time reverse location method in a
volcanic setting, for a family of longperiod events recorded on Mt
Etna. Results are compared with locations determined using a full
moment tensor grid search inversion and crosscorrelation method.
From June 18th to July 3rd, 2008, 50 broadband
seismic stations were deployed on Mt Etna, Italy, in close proximity
to the summit. Two families of longperiod events were detected with
dominant spectral peaks around 0.9 Hz. The large number of stations
close to the summit allowed us to locate all events in both families
using a time reversal location method. The method involves taking the
seismic signal, reversing it in time, and using it as a seismic
source in a numerical seismic wave simulator where the reversed
signals propagate through the numerical model, interfere
constructively and destructively, and focus on the original source
location. The source location is the computational cell with the
largest displacement magnitude at the time of maximum energy current
density inside the grid. Before we located the two longperiod
families we first applied the method to two synthetic datasets and
found a good fit between the time reverse location and true synthetic
location for a known velocity model. The time reverse location
results of the two families show a shallow seismic region close to
the summit in agreement with the locations using a moment tensor full
waveform inversion method and a crosscorrelation location method.
20110101T00:00:00ZModelling fluid induced seismicity on a nearby active faultMurphy, ShaneO'Brien, G. S.McCloskey, J.Bean, Christopher J.Nalbant, S.http://hdl.handle.net/10197/543020140228T09:17:16Z20130901T00:00:00ZModelling fluid induced seismicity on a nearby active fault
Murphy, Shane; O'Brien, G. S.; McCloskey, J.; Bean, Christopher J.; Nalbant, S.
We present a numerical investigation of the effect that static stress perturbations due to fluid
injection have on a nearby active fault where the fluid does not come in physical contact
with the fault. Our modelling employs a lattice Boltzmann pore diffusion model coupled
with a quasidynamic earthquake rupture model. As diffusivities and frictional parameters can
be defined independently at individual nodes/cells this allows us to replicate complex 3D
geological media in our simulations. We demonstrate the effect an injection can have on an
active nearby fault. Compared with our control catalogue (identical to the original simulation
but without the injection), the injection not only altered the timing of the next earthquake
sequence, it also changed its size, producing a Mw 6.7 event, the largest observed earthquake
on the fault. This large event pushes the fault into a subcritical state from which it took roughly
200 yr of continuous tectonic loading for the fault to return to a critical state.
20130901T00:00:00ZThe coupling between very long period seismic events, volcanic tremor, and degassing rates at Mount Etna volcanoZuccarello, LucianoBurton, Michael R.Saccorotti, GilbertoBean, Christopher J.Patané, Domenicohttp://hdl.handle.net/10197/542820140320T04:00:14Z20130901T00:00:00ZThe coupling between very long period seismic events, volcanic tremor, and degassing rates at Mount Etna volcano
Zuccarello, Luciano; Burton, Michael R.; Saccorotti, Gilberto; Bean, Christopher J.; Patané, Domenico
From December 2005 to January 2006, an anomalous degassing episode was observed
at Mount Etna, wellcorrelated with an increase in volcanic tremor, and in the almost
complete absence of eruptive activity. In the same period, more than 10,000 very long
period (VLP) events were detected. Through moment tensor inversion analyses of the VLP
pulses, we obtained quantitative estimates of the volumetric variations associated with these
events. This allowed a quantitative investigation of the relationship between VLP seismic
activity, volcanic tremor, and gas emission rate at Mount Etna. We found a statistically
significant positive correlation between SO2 gas flux and volcanic tremor, suggesting that
tremor amplitude can be used as a firstorder proxy for the background degassing activity of
the volcano. VLP volumetric changes and SO2 gas flux are correlated only for the last part of
our observations, following a slight change in the VLP source depth. We calculate that the
gas associated with VLP signal genesis contributed less than 5% of the total gas emission.
The existence of a linear correlation between VLP and degassing activities indicates a
general relationship between these two processes. The effectiveness of such coupling
appears to depend upon the particular location of the VLP source, suggesting that conduit
geometry might play a significant role in the VLPgenerating process. These results are the
first report on Mount Etna of a quantitative relationship between the amounts of gas
emissions directly estimated through instrumental flux measurements and the quantities of
gas mass inferred in the VLP source inversion.
20130901T00:00:00Z