Browsing by Author "Ceruso, Mariangela"

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Mechanism of the binding of Z-L-tryptophan and Z-L-phenylalanine to thermolysin and stromelysin-1 in aqueous solutions

Ceruso, Mariangela ; Howe, Nicole ; Malthouse, J.Paul G. (Elsevier B.V., 2012-02)

The chemical shift of the carboxylate carbon of Z-tryptophan is increased from 179.85 to 182.82 ppm and 182.87 on binding to thermolysin and stromelysin-1 respectively. The chemical shift of Z-phenylalanine is also increased ...
pH stability of the stromelysin-1 catalytic domain and its mechanism of interaction with a glyoxal inhibitor

Howe, Nicole ; Ceruso, Mariangela ; Spink, Edward ; Malthouse, J.Paul G. (Elsevier, 2011-10)

The Stromelysin-1 catalytic domain83-247 (SCD) is stable for at least 16 hours at pHs 6.0-8.4. At pHs 5.0 and 9.0 there is exponential irreversible denaturation with half lives of 38 and 68 min respectively. At pHs 4.5 ...